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positive displacement syringe pumps  (Harvard Bioscience)
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a Schematic diagram of eDIBs and key bilayer interfaces before (−LPC) and after (+LPC) the addition of lysolipids. i The eDIB system and bilayer interfaces are at equilibrium, as attractive and repulsive forces balance each other. ii The introduced lysolipids take the eDIB out of equilibrium, as the LPC and DOPC contribute to the lateral expansion of the droplet-hydrogel DIB, by inserting from the external and internal side of the bilayer, respectively. Consequently, the attractive forces parallel to the droplet-hydrogel DIB \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\,\left({\bar{F}}_{{dh}-{att}}\right)$$\end{document} F ¯ d h − a t t rise, due to the tensional changes at the outer bilayer. The contact angle ( θ b ) between the droplets is influenced by the increasing attractive forces at the droplet-hydrogel DIB. b Time-lapse of the inner aqueous droplets of eDIBs treated with 1 μΜ and 10 μΜ LPC, showing significant pulling and subsequent merging of droplets treated with 10 μM LPC. c Plots of the, (i) X and Y position of the inner droplets and, (ii) the mean square <t>displacement</t> (MSD) of 0 μM, 1 μM and 10 μM LPC treated eDIBs measured over 11 h, revealing that 1 μM treated droplets traveled similarly to the untreated construct, while there was significant travel by 10 μM treated droplets. The dots in (i). show the location of the individual droplets at t = 0. Error bars in (ii). correspond to the standard error of the mean (±SEM).
Positive Displacement Syringe Pumps, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Schematic diagram of eDIBs and key bilayer interfaces before (−LPC) and after (+LPC) the addition of lysolipids. i The eDIB system and bilayer interfaces are at equilibrium, as attractive and repulsive forces balance each other. ii The introduced lysolipids take the eDIB out of equilibrium, as the LPC and DOPC contribute to the lateral expansion of the droplet-hydrogel DIB, by inserting from the external and internal side of the bilayer, respectively. Consequently, the attractive forces parallel to the droplet-hydrogel DIB \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\,\left({\bar{F}}_{{dh}-{att}}\right)$$\end{document} F ¯ d h − a t t rise, due to the tensional changes at the outer bilayer. The contact angle ( θ b ) between the droplets is influenced by the increasing attractive forces at the droplet-hydrogel DIB. b Time-lapse of the inner aqueous droplets of eDIBs treated with 1 μΜ and 10 μΜ LPC, showing significant pulling and subsequent merging of droplets treated with 10 μM LPC. c Plots of the, (i) X and Y position of the inner droplets and, (ii) the mean square <t>displacement</t> (MSD) of 0 μM, 1 μM and 10 μM LPC treated eDIBs measured over 11 h, revealing that 1 μM treated droplets traveled similarly to the untreated construct, while there was significant travel by 10 μM treated droplets. The dots in (i). show the location of the individual droplets at t = 0. Error bars in (ii). correspond to the standard error of the mean (±SEM).
Positive Displacement Syringe Pump, supplied by Pump Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive displacement syringe pump  (Harvard Bioscience)
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a Schematic diagram of eDIBs and key bilayer interfaces before (−LPC) and after (+LPC) the addition of lysolipids. i The eDIB system and bilayer interfaces are at equilibrium, as attractive and repulsive forces balance each other. ii The introduced lysolipids take the eDIB out of equilibrium, as the LPC and DOPC contribute to the lateral expansion of the droplet-hydrogel DIB, by inserting from the external and internal side of the bilayer, respectively. Consequently, the attractive forces parallel to the droplet-hydrogel DIB \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\,\left({\bar{F}}_{{dh}-{att}}\right)$$\end{document} F ¯ d h − a t t rise, due to the tensional changes at the outer bilayer. The contact angle ( θ b ) between the droplets is influenced by the increasing attractive forces at the droplet-hydrogel DIB. b Time-lapse of the inner aqueous droplets of eDIBs treated with 1 μΜ and 10 μΜ LPC, showing significant pulling and subsequent merging of droplets treated with 10 μM LPC. c Plots of the, (i) X and Y position of the inner droplets and, (ii) the mean square <t>displacement</t> (MSD) of 0 μM, 1 μM and 10 μM LPC treated eDIBs measured over 11 h, revealing that 1 μM treated droplets traveled similarly to the untreated construct, while there was significant travel by 10 μM treated droplets. The dots in (i). show the location of the individual droplets at t = 0. Error bars in (ii). correspond to the standard error of the mean (±SEM).
Positive Displacement Syringe Pump, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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positive displacement microprocessor syringe pump  (Harvard Bioscience)
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a Schematic diagram of eDIBs and key bilayer interfaces before (−LPC) and after (+LPC) the addition of lysolipids. i The eDIB system and bilayer interfaces are at equilibrium, as attractive and repulsive forces balance each other. ii The introduced lysolipids take the eDIB out of equilibrium, as the LPC and DOPC contribute to the lateral expansion of the droplet-hydrogel DIB, by inserting from the external and internal side of the bilayer, respectively. Consequently, the attractive forces parallel to the droplet-hydrogel DIB \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\,\left({\bar{F}}_{{dh}-{att}}\right)$$\end{document} F ¯ d h − a t t rise, due to the tensional changes at the outer bilayer. The contact angle ( θ b ) between the droplets is influenced by the increasing attractive forces at the droplet-hydrogel DIB. b Time-lapse of the inner aqueous droplets of eDIBs treated with 1 μΜ and 10 μΜ LPC, showing significant pulling and subsequent merging of droplets treated with 10 μM LPC. c Plots of the, (i) X and Y position of the inner droplets and, (ii) the mean square <t>displacement</t> (MSD) of 0 μM, 1 μM and 10 μM LPC treated eDIBs measured over 11 h, revealing that 1 μM treated droplets traveled similarly to the untreated construct, while there was significant travel by 10 μM treated droplets. The dots in (i). show the location of the individual droplets at t = 0. Error bars in (ii). correspond to the standard error of the mean (±SEM).
Positive Displacement Microprocessor Syringe Pump, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/positive+displacement+syringe/microprocessor+controlled+syringe+pump/10__1002_slash_aelm__202001213-257-9-14
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positive displacement syringe  (Hamilton Company)
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Hamilton Company positive displacement syringe
a Schematic diagram of eDIBs and key bilayer interfaces before (−LPC) and after (+LPC) the addition of lysolipids. i The eDIB system and bilayer interfaces are at equilibrium, as attractive and repulsive forces balance each other. ii The introduced lysolipids take the eDIB out of equilibrium, as the LPC and DOPC contribute to the lateral expansion of the droplet-hydrogel DIB, by inserting from the external and internal side of the bilayer, respectively. Consequently, the attractive forces parallel to the droplet-hydrogel DIB \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\,\left({\bar{F}}_{{dh}-{att}}\right)$$\end{document} F ¯ d h − a t t rise, due to the tensional changes at the outer bilayer. The contact angle ( θ b ) between the droplets is influenced by the increasing attractive forces at the droplet-hydrogel DIB. b Time-lapse of the inner aqueous droplets of eDIBs treated with 1 μΜ and 10 μΜ LPC, showing significant pulling and subsequent merging of droplets treated with 10 μM LPC. c Plots of the, (i) X and Y position of the inner droplets and, (ii) the mean square <t>displacement</t> (MSD) of 0 μM, 1 μM and 10 μM LPC treated eDIBs measured over 11 h, revealing that 1 μM treated droplets traveled similarly to the untreated construct, while there was significant travel by 10 μM treated droplets. The dots in (i). show the location of the individual droplets at t = 0. Error bars in (ii). correspond to the standard error of the mean (±SEM).
Positive Displacement Syringe, supplied by Hamilton Company, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a Schematic diagram of eDIBs and key bilayer interfaces before (−LPC) and after (+LPC) the addition of lysolipids. i The eDIB system and bilayer interfaces are at equilibrium, as attractive and repulsive forces balance each other. ii The introduced lysolipids take the eDIB out of equilibrium, as the LPC and DOPC contribute to the lateral expansion of the droplet-hydrogel DIB, by inserting from the external and internal side of the bilayer, respectively. Consequently, the attractive forces parallel to the droplet-hydrogel DIB \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\,\left({\bar{F}}_{{dh}-{att}}\right)$$\end{document} F ¯ d h − a t t rise, due to the tensional changes at the outer bilayer. The contact angle ( θ b ) between the droplets is influenced by the increasing attractive forces at the droplet-hydrogel DIB. b Time-lapse of the inner aqueous droplets of eDIBs treated with 1 μΜ and 10 μΜ LPC, showing significant pulling and subsequent merging of droplets treated with 10 μM LPC. c Plots of the, (i) X and Y position of the inner droplets and, (ii) the mean square displacement (MSD) of 0 μM, 1 μM and 10 μM LPC treated eDIBs measured over 11 h, revealing that 1 μM treated droplets traveled similarly to the untreated construct, while there was significant travel by 10 μM treated droplets. The dots in (i). show the location of the individual droplets at t = 0. Error bars in (ii). correspond to the standard error of the mean (±SEM).

Journal: Communications Chemistry

Article Title: Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations

doi: 10.1038/s42004-024-01209-z

Figure Lengend Snippet: a Schematic diagram of eDIBs and key bilayer interfaces before (−LPC) and after (+LPC) the addition of lysolipids. i The eDIB system and bilayer interfaces are at equilibrium, as attractive and repulsive forces balance each other. ii The introduced lysolipids take the eDIB out of equilibrium, as the LPC and DOPC contribute to the lateral expansion of the droplet-hydrogel DIB, by inserting from the external and internal side of the bilayer, respectively. Consequently, the attractive forces parallel to the droplet-hydrogel DIB \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\,\left({\bar{F}}_{{dh}-{att}}\right)$$\end{document} F ¯ d h − a t t rise, due to the tensional changes at the outer bilayer. The contact angle ( θ b ) between the droplets is influenced by the increasing attractive forces at the droplet-hydrogel DIB. b Time-lapse of the inner aqueous droplets of eDIBs treated with 1 μΜ and 10 μΜ LPC, showing significant pulling and subsequent merging of droplets treated with 10 μM LPC. c Plots of the, (i) X and Y position of the inner droplets and, (ii) the mean square displacement (MSD) of 0 μM, 1 μM and 10 μM LPC treated eDIBs measured over 11 h, revealing that 1 μM treated droplets traveled similarly to the untreated construct, while there was significant travel by 10 μM treated droplets. The dots in (i). show the location of the individual droplets at t = 0. Error bars in (ii). correspond to the standard error of the mean (±SEM).

Article Snippet: Each liquid phase was delivered to the microfluidic device using SGE gas-tight glass syringes loaded onto positive displacement syringe pumps (KD Scientific).

Techniques: Construct